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KMID : 0900920020260030215
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Korean journal of Animal Reproduction
2002 Volume.26 No. 3 p.215 ~ p.221
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Effect of Antioxidants Plus Growth Factors on In Vitro Development of Porcine IVM/IVF Embryos
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Choi Young-Jin
Park Choon-Keun
Cheong Hee-Tae
Kim Choung-Ik
Park Dong-Heon
Jang Hyun-Yong
Park Jin-Ki
Yang Boo-Keun
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Abstract
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Antioxidants(N-acetyl-1-cysteine, ebselen and glutathione) and growth factors(EGF, PDGF) were studied as a mean of increasing the development of porcine embryos produced by in vitro maturation (IVM) and in vitro fertilization(IVF). Porcine embryos developed to the 2~8 cell stage after IVF were cultured fer 6 to 7 days at 38.5^{circ}C in NCSU 23 medium containing antioxidants plus growth factors. Cell numbers of blastocysts were counted by fluorescence staining method. The developmental rate beyond morula stages in NCSU 23 containing NAC(1nm) or NAC(1nm) plus EGF(100ng/mell) or PDGF(5ng/mell) were 28.1, 32.3 and 35.3%, respectively. NAC plus PDGF group was slightly higher than control group(P>0.05). The developmental capacity in NCSU 23 containing ebselen(10 muM) or ebselen(10 muM) plus EGF(100ng/mell) or PDGF(5ng/mell) were 17.8, 36.9 and 40.3%, respectively Ebselen plus growth factor groups were significantly higher than control group(P<0.05). The developmental capacity in NCSU 23 containing glutathione(100 muM) or glutathione(100 muM) plus EGF (100ng/mell) or PDGF(5ng/mell) were 24.1, 30.5 and 27.7%, respectively. There were not difference in all experimental groups(P>0.05). In all experimental groups, there was no significantly differences on the cell number of blastocysts, but ebselen plus growth factor groups were significantly higher than control group. These studies indicate that antioxidants plus growth factors can increase the proportion of embryos that developed beyond morulae stage.
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KEYWORD
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Antioxidant, Growth factor, Porcine embryo
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